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PCR amplification of Ghisp20 gene :

The DNA sequence of Ghisp20 was amplified by PCR Q5 to generate fragment allowing a SLIC cloning. The expected size is 570bp and a +/- 600bp band is observed. Amplification of DNAs sequences with primers was validated.

Figure. Verification PCR amplification of DNAs sequence and these extensions for SLIC cloning. a. Control – (Absence of DNAs, no band expected); b, c, d, e. Amplification of 6hisp20_flagcry11Aa (expected 2500 bp), flagcry11Aa (expected 1847 bp), strepcyt1Aa (expected 775 bp) and 6hisp20 (expected 568 bp); L (SMART Ladder, its size is shown on the right side).

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